CovidV1, Main, Exploration, bibRecord, 000F94

Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections

Identifieur interne : 000F94 ( Main/Exploration ); précédent : 000F93; suivant : 000F95

Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections

Auteurs : Aleksandar Radoni [Allemagne] ; Stefanie Thulke [Allemagne] ; Hi-Gung Bae [Allemagne] ; Marcel A. Müller [Allemagne] ; Wolfgang Siegert [Allemagne] ; Andreas Nitsche [Allemagne]

Source :

Virology Journal [ 1743-422X ] ; 2005.

RBID : PMC:549079

Descripteurs français

English descriptors

KwdEn :
- Cell Line, Cytomegalovirus (genetics), Cytomegalovirus (isolation & purification), Genes, Viral (genetics), Herpesvirus 6, Human (genetics), Herpesvirus 6, Human (isolation & purification), Humans, Peptidylprolyl Isomerase (genetics), Peptidylprolyl Isomerase (metabolism), Polymerase Chain Reaction (methods), Polymerase Chain Reaction (standards), Poxviridae (genetics), Poxviridae (isolation & purification), Reference Standards, SARS Virus (genetics), SARS Virus (isolation & purification), TATA-Box Binding Protein (metabolism), Virus Replication, Yellow fever virus (genetics), Yellow fever virus (isolation & purification).
MESH :
- chemical , genetics : Peptidylprolyl Isomerase.
- genetics : Cytomegalovirus, Genes, Viral, Herpesvirus 6, Human, Poxviridae, SARS Virus, Yellow fever virus.
- isolation & purification : Cytomegalovirus, Herpesvirus 6, Human, Poxviridae, SARS Virus, Yellow fever virus.
- chemical , metabolism : Peptidylprolyl Isomerase, TATA-Box Binding Protein.
- methods : Polymerase Chain Reaction.
- standards : Polymerase Chain Reaction.
- Cell Line, Humans, Reference Standards, Virus Replication.

Abstract

Ten potential reference genes were compared for their use in experiments investigating cellular mRNA expression of virus infected cells. Human cell lines were infected with Cytomegalovirus, Human Herpesvirus-6, Camelpox virus, SARS coronavirus or Yellow fever virus. The expression levels of these genes and the viral replication were determined by real-time PCR. Genes were ranked by the BestKeeper tool, the GeNorm tool and by criteria we reported previously. Ranking lists of the genes tested were tool dependent. However, over all, β-actin is an unsuitable as reference gene, whereas TATA-Box binding protein and peptidyl-prolyl-isomerase A are stable reference genes for expression studies in virus infected cells.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC549079

DOI: 10.1186/1743-422X-2-7
PubMed: 15705200
PubMed Central: 549079

Affiliations:

Le document en format XML

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<term>Herpesvirus 6, Human (isolation & purification)</term>
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<term>Virus de la fièvre jaune (isolement et purification)</term>
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<term>Virus du SRAS (isolement et purification)</term>
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<term>Herpesvirus 6, Human</term>
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<term>Lignée cellulaire</term>
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<front><div type="abstract" xml:lang="en"><p>Ten potential reference genes were compared for their use in experiments investigating cellular mRNA expression of virus infected cells. Human cell lines were infected with Cytomegalovirus, Human Herpesvirus-6, Camelpox virus, SARS coronavirus or Yellow fever virus. The expression levels of these genes and the viral replication were determined by real-time PCR. Genes were ranked by the BestKeeper tool, the GeNorm tool and by criteria we reported previously. Ranking lists of the genes tested were tool dependent. However, over all, β-actin is an unsuitable as reference gene, whereas TATA-Box binding protein and peptidyl-prolyl-isomerase A are stable reference genes for expression studies in virus infected cells.</p>
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Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections

Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

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